The appeal by NHS fundraiser Colonel Tom Moore topped £32 million as it closed at midnight.
The Second World War veteran celebrated his 100th birthday on Thursday, just a few weeks after he first set out to walk 100 laps of his garden with a fundraising target of £1,000.
But his determination captured the public's heart and by the time his JustGiving page closed at midnight on Thursday, he had raised a staggering £32,794,651.
Donations have now been closed on the page, but the total amount of cash raised was still rising as donations continued to be processed at the time of writing.
Colonel Tom has received thousands of cards from well-wishers, including the Queen, and got a birthday message from the Prime Minister.
To mark his century he was made an honorary colonel and there were two military flypasts over his village – the first of a Spitfire and a Hurricane, and the second of a Wildcat and an Apache helicopter.
Captain Tom Moore's 100th Birthday Flypast - In pictures
He has been sent more than 140,000 birthday cards, including a signed card from the Queen, and was sent a message by Prime Minister Boris Johnson.
Mr Johnson said: “Captain Tom, I know I speak for the whole country when I say ‘We wish you a very happy 100th birthday’.
“Your heroic efforts have lifted the spirits of the entire nation.”
Colonel Tom, who is a cricket fan, has also been made an honorary member of the England cricket team.
He began his challenge to thank NHS staff who helped him after he was treated for a broken hip, and he thanked the public for their “very overwhelming” generosity.
“Reaching 100 is quite something,” he said.
“Reaching 100 with such interest in me and huge generosity from the public is very overwhelming.
“People keep saying what I have done is remarkable, however it’s actually what you have done for me which is remarkable.
“I felt a little frustrated and disappointed after I broke my hip and it knocked my confidence.
“However, the past three weeks have put a spring back in my step."
Captain Tom added that the success of his fundraising campaign had given him renewed purpose.
“I have renewed purpose and have thoroughly enjoyed every second of this exciting adventure, but I can’t keep walking forever", he said.
“My legs may be tired, but my mind is racing and I’m hoping to be back very soon with other ways in which I can help people, help others.
“Please always remember ‘Tomorrow Will Be A Good Day’.”
